Biological activity of plumbago scandens L. against multidrug-resistance strains of Mycobacterium tuberculosis

Translated title of the contribution: Biological activity of plumbago scandens L. against multidrug-resistance strains of Mycobacterium tuberculosis

Natali Moncada Ascencio, Manuel Farcio Villarreal, Consuelo Rojas Idrogo, Dalva Trevisan Ferreira, Oscar Horna Dávila, Jurandir Pereira, Guillermo E. Delgado Paredes

Research output: Contribution to journalArticlepeer-review

Abstract

Tuberculosis control is a high priority for the Ministry of Health from Peru. In the present work the inhibitory effect of both metanolic (MeOH) and ethyl acetate (AcOEt) crude extracts and the minimum inhibitory concentration (MIC) of both wild plants and in vitro plantlets of Plumbago scandens L.-Plumbaginaceae-against multidrug-resistance (MDR) strains of Mycobacterium tuberculosis were determined. The plant material was constituted by roots and seeds, collected in the Motupe (Lambayeque) area. The in vitro plantlets were obtained from seedlings and micropropagated by shoot tips and nodal segments in Murashige and Skoog (MS) culture medium. The microbiological material consisting of one control strain and two strains of Mycobacterium tuberculosis resistant to isoniazid (INH) and rifampicin (RIF) was cultivated using the Lowenstein-Jensen culture medium. The MIC values varied from 0,65 to 1,3 mg/mL. Bacterial strains showed more sensitivite to the AcOEt crude extract. The GC analysis of the plant material showed the presence of the naphtoquinone plumbagin and other aromatic compounds. In conclusion, the MeOH y AcOEt crude extracts from roots of the wild plants and MeOH crude extract of in vitro plantlets of P. scandens showed a strong inhibitory activity against MDR strains of M. tuberculosis.

Translated title of the contributionBiological activity of plumbago scandens L. against multidrug-resistance strains of Mycobacterium tuberculosis
Original languageEnglish
Pages (from-to)233-245
Number of pages13
JournalBoletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas
Volume10
Issue number3
StatePublished - May 2011

Keywords

  • Antimicrobial activity
  • Metanolic extract
  • Plumbagin
  • Tissue culture
  • Tuberculosis

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