TY - JOUR
T1 - Biological activity of plumbago scandens L. against multidrug-resistance strains of Mycobacterium tuberculosis
AU - Moncada Ascencio, Natali
AU - Farcio Villarreal, Manuel
AU - Rojas Idrogo, Consuelo
AU - Trevisan Ferreira, Dalva
AU - Horna Dávila, Oscar
AU - Pereira, Jurandir
AU - Delgado Paredes, Guillermo E.
PY - 2011/5
Y1 - 2011/5
N2 - Tuberculosis control is a high priority for the Ministry of Health from Peru. In the present work the inhibitory effect of both metanolic (MeOH) and ethyl acetate (AcOEt) crude extracts and the minimum inhibitory concentration (MIC) of both wild plants and in vitro plantlets of Plumbago scandens L.-Plumbaginaceae-against multidrug-resistance (MDR) strains of Mycobacterium tuberculosis were determined. The plant material was constituted by roots and seeds, collected in the Motupe (Lambayeque) area. The in vitro plantlets were obtained from seedlings and micropropagated by shoot tips and nodal segments in Murashige and Skoog (MS) culture medium. The microbiological material consisting of one control strain and two strains of Mycobacterium tuberculosis resistant to isoniazid (INH) and rifampicin (RIF) was cultivated using the Lowenstein-Jensen culture medium. The MIC values varied from 0,65 to 1,3 mg/mL. Bacterial strains showed more sensitivite to the AcOEt crude extract. The GC analysis of the plant material showed the presence of the naphtoquinone plumbagin and other aromatic compounds. In conclusion, the MeOH y AcOEt crude extracts from roots of the wild plants and MeOH crude extract of in vitro plantlets of P. scandens showed a strong inhibitory activity against MDR strains of M. tuberculosis.
AB - Tuberculosis control is a high priority for the Ministry of Health from Peru. In the present work the inhibitory effect of both metanolic (MeOH) and ethyl acetate (AcOEt) crude extracts and the minimum inhibitory concentration (MIC) of both wild plants and in vitro plantlets of Plumbago scandens L.-Plumbaginaceae-against multidrug-resistance (MDR) strains of Mycobacterium tuberculosis were determined. The plant material was constituted by roots and seeds, collected in the Motupe (Lambayeque) area. The in vitro plantlets were obtained from seedlings and micropropagated by shoot tips and nodal segments in Murashige and Skoog (MS) culture medium. The microbiological material consisting of one control strain and two strains of Mycobacterium tuberculosis resistant to isoniazid (INH) and rifampicin (RIF) was cultivated using the Lowenstein-Jensen culture medium. The MIC values varied from 0,65 to 1,3 mg/mL. Bacterial strains showed more sensitivite to the AcOEt crude extract. The GC analysis of the plant material showed the presence of the naphtoquinone plumbagin and other aromatic compounds. In conclusion, the MeOH y AcOEt crude extracts from roots of the wild plants and MeOH crude extract of in vitro plantlets of P. scandens showed a strong inhibitory activity against MDR strains of M. tuberculosis.
KW - Antimicrobial activity
KW - Metanolic extract
KW - Plumbagin
KW - Tissue culture
KW - Tuberculosis
UR - http://www.scopus.com/inward/record.url?scp=79958703002&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:79958703002
SN - 0717-7917
VL - 10
SP - 233
EP - 245
JO - Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas
JF - Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas
IS - 3
ER -