Actividad tripanocida de piper solmsianum C. DC. Sobre formas epimastigota y tripomastigota de trypanosoma cruzi

Introduction: the infection by Trypanosoma cruzi, known as Chagas’ disease, poses a major public health problem in Central and South America countries. Objective: to evaluate the activity of crude ethyl acetate extracts from in vitro plants of 6-8 and 10-12 months of age, stem barks and mature wild plant leaves and tetrahydrofuran lignin grandisin isolated from Piper solmsianum against the epimastigote and trypomastigote forms of T. cruzi in vitro. Methods: in the evaluation of the effect of various crude ethyl acetate extracts and grandisin from P. solmsianum on the viability of epimastigote and trypomastigote forms of T. cruzi, the MTT method (3-(4,5-dimethylthiazol-2-il)-2,5-diphenyltetrazolium bromide) was used. Results: in the epimastigote form, the best results in growth inhibition was obtained with 50 µg/mL of stem extract, and in the trypomastigote form, with 25 and 50 µg/mL of grandisin and 6-8 months-old in vitro plants, respectively. The inhibition values in all cases ranged from 86 to 96%. 6-8 months old in vitro plants and grandisin were found to be active against the epimastigote and trypomastigote forms of T. cruzi with IC₅₀ of 0.018 µg/mL and 0.360 µg/mL, respectively. Conclusions: the trypanocidal activity of extracts from wild plants and in vitro plants of P. solmsianum was proved.